Composite mantle cell and Burkitt lymphoma: a rare case report

Composite lymphoma (CL) is a rare entity defined by two or more morphologically distinct types of lymphoma (Hodgkin [HL] and non-Hodgkin lymphoma [NHL], B-cell lymphoma, T-cell lymphoma, or a mixture of B-cell and T-cell) within a single organ or tissue. The definition of CL is a matter of debate because it is difficult to prove simultaneous occurrence of two unrelated lymphomas and that CL is not just a clonal evolution such as histologic transformation, whose treatment approach is doubtful but are essentially targeted against the high-grade component.1 This case report, as far as we know, describes the first case of CL of Burkitt and mantle cell lymphoma. Composite lymphoma is a rare entity defined by two or more morphologically distinct types of lymphoma (HL and NHL, B-cell lymphoma, T-cell lymphoma, or a mixture of B-cell and T-cell) within a single organ or tissue. 2 Most cases of CL, the exact etiology of which is unknown, 1,3 are two distinct types of B-cell lymphomas (usually a low-grade B-cell lymphoma), whose treatment approach is doubtful but are essentially targeted against the high-grade component.1 This case report, as far as we know, describes the first case of CL of Burkitt and mantle cell lymphoma. A 68-year-old man presented with complaints of swelling in the right leg a month ago. The patient denied other symptoms, including B symptoms. Physical examination showed cervical and axillary adenopathies and a large inguinal mass associated with marked swelling of the right leg and scrotum. A computed tomography (CT) scan revealed supra and infradiaphragmatic lymph nodes, the largest lymph nodes involving the

Composite lymphoma (CL) is a rare entity defined by two or more morphologically distinct types of lymphoma (Hodgkin [HL] and non-Hodgkin lymphoma [NHL], B-cell lymphoma, T-cell lymphoma, or a mixture of B-cell and T-cell) within a single organ or tissue.The definition of CL is a matter of debate because it is difficult to prove simultaneous occurrence of two unrelated lymphomas and that CL is not just a clonal evolution such as histologic transformation, whose treatment approach is doubtful but are essentially targeted against the high-grade component.1This case report, as far as we know, describes the first case of CL of Burkitt and mantle cell lymphoma.
Composite lymphoma is a rare entity defined by two or more morphologically distinct types of lymphoma (HL and NHL, B-cell lymphoma, T-cell lymphoma, or a mixture of B-cell and T-cell) within a single organ or tissue. 2 Most cases of CL, the exact etiology of which is unknown, 1,3 are two distinct types of B-cell lymphomas (usually a low-grade B-cell lymphoma), whose treatment approach is doubtful but are essentially targeted against the high-grade component.1This case report, as far as we know, describes the first case of CL of Burkitt and mantle cell lymphoma.
A 68-year-old man presented with complaints of swelling in the right leg a month ago.The patient denied other symptoms, including B symptoms.Physical examination showed cervical and axillary adenopathies and a large inguinal mass associated with marked swelling of the right leg and scrotum.A computed tomography (CT) scan revealed supra and infradiaphragmatic lymph nodes, the largest lymph nodes involving the right iliac obturator with 14 cm.Initial blood work showed a Hb of 14.8 g/ dL, WBC of 8.21 3 10 9 /L with a normal differential and a Plt of 237 3 10 9 /L, normal renal and hepatic function, and lactate dehydrogenase level of 216 U/L.
Before the initial treatment with chemotherapy R-CHOP protocol (rituximab, cyclophosphamide, vincristine, doxorubicin, and prednisolone), the patient was diagnosed with SARS-CoV-2 infection, without symptoms, and postponed the treatment one month.
After two cycles of R-CHOP protocol without clinical response, the treatment was suspended and a second line with R-HyperCVAD protocol (alternate cycles of a chemotherapy drug combination of rituximab, cyclophosphamide, doxorubicin, vincristine and prednisone, and methotrexate and cytarabine) was initiated.After the 3rd cycle, an atrial fibrillation with heart failure was diagnosed and the treatment was suspended.A bone marrow evaluation was performed and did not show any evidence of lymphoproliferative disorder, and a PET-CT did not show FDG-avid lesions.The patient is currently in clinical surveillance without clinical symptoms.
This case reports the first documented composite Burkitt and mantle cell lymphoma.The majority of components of CL were clonally related, suggesting a common origin. 4,5In fact, the definition of CL is a matter of debate because it is difficult to prove simultaneous occurrence of two unrelated lymphomas and that CL is not just a clonal evolution such as histologic transformation (eg.FL or CLL to DLBCL). 3,6In our case, aside the different morphologies in two components, the FISH analysis of the mantle cell component was negative for rearrangement of the BCL6, MYC, or BCL2 genes and the Burkitt component was negative for the BCL6, BCL2, and CCND1/IGH rearrangements.This result supports the co-existence of two cytogenetically independent lymphomas.
Management of CL is extremely challenging, as there is no known standard of care treatment.In our case report, there was not any response with R-CHOP protocol, but the clinical and imaging improvement with addition of cytarabine and methotrexate highlights that HyperCVAD protocol appears to be an effective choice for CL of Burkitt and mantle cell lymphoma.However, long-term follow-up is necessary in our case to determine the outcome of this treatment.

Figure 1 .
Figure 1.(A) (H&E 40X) biopsy sample from right inguinal mass showing two distinct cell populations: (B) (H&E 200X) on the upper half of the picture, there is a predominance of small cells, with centrocyte-like appearance, consistent with mantle cell lymphoma.

Figure 2 .
Figure 2. (C) (H&E 200X) on the lower half, the cell population is mostly comprised medium size cells with round nuclei containing multiple small nucleoli and apoptosis, compatible with Burkitt lymphoma.(D-G) Immunohistochemistry highlighting the main differences between the two components (D-CD20, E-Bcl-2, F-cyclin D1, G-CD10).FISH analysis showing IGH-CCND1 fusion signals (yellow) in the MCL component (H) and separate red and green signals in the Burkitt component with c-MYC rearrangement (I).